tag:blogger.com,1999:blog-61099925313740284452024-03-06T00:16:38.341-08:00On The Science HorizonAs a family-owned certified small business, our Mission is simple:
We offer only top-rated quality products and a dedication to the highest level of customer service delivered with a sense of warmth, friendliness, individual pride, and company spirit.
Visit our website www.midsci.com for monthly specials news and outstanding products!Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.comBlogger69125tag:blogger.com,1999:blog-6109992531374028445.post-13745687029639580402015-04-20T11:13:00.000-07:002015-04-20T11:13:10.503-07:00YOU NEED TO PASS YOUR DMRQA… THE DATE IS RAPIDLY APPROACHING…WANT A LITTLE HELP?<div class="Default" style="margin-top: .35pt;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">You’re in charge of the Membrane Filtration Station for
microbiology for the DMRQA test this year. <o:p></o:p></span></div>
<div class="Default" style="margin-bottom: .0001pt; margin-bottom: 0in; margin-left: 28.0pt; margin-right: -2.7pt; margin-top: 1.65pt; text-align: justify;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">It’s a big responsibility, and
demands your fullest attention. <o:p></o:p></span></div>
<div class="Default" style="margin-bottom: .0001pt; margin-bottom: 0in; margin-left: 28.0pt; margin-right: -2.7pt; margin-top: 0in; text-align: justify;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Maybe you’re a new technician, or
maybe the person who ,”always does it” isn’t around anymore. What if, you don’t
pass, and wouldn’t it be great to have another guide? <o:p></o:p></span></div>
<div class="MsoNormal">
This short informative report will help give you some
guidance to assure you’re on the road to success when it comes to testing using
membrane filtration.<o:p></o:p></div>
<div class="Default">
<br /></div>
<div class="Default" style="margin-top: .35pt;">
<b><i><span style="font-size: 14.0pt;">ONE: </span></i></b><span style="font-size: 14.0pt;"><o:p></o:p></span></div>
<div class="Default">
<span style="font-size: 11.5pt;">THE 5 “P’S” – LET’S START
THERE! <o:p></o:p></span></div>
<div class="Default" style="margin-bottom: .0001pt; margin-bottom: 0in; margin-left: 29.6pt; margin-right: 187.25pt; margin-top: 0in; text-align: justify;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">You’ve heard it before: <o:p></o:p></span></div>
<div class="Default" style="margin-bottom: .0001pt; margin-bottom: 0in; margin-left: 29.6pt; margin-right: 22.85pt; margin-top: 0in; text-align: justify;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">“Proper preparation prevents poor
performance.” Sometimes with colorful language added…but nevertheless it’s a
phrase that is worth repeating! <o:p></o:p></span></div>
<div class="MsoNormal">
When we talk about being properly prepared in membrane
filtration, it begins at the sink. You want to make sure that the filter
funnels are nice and clean and free from any debris. They should also be
checked periodically for high or low pH walls with a pH indicator solution like
bromothymol blue. Make sure all filter funnels, magnetic bases, and manifold
are scrupulously clean.<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="Default">
<br /></div>
<div class="Default">
<b><i><span style="font-size: 11.5pt;">TWO: </span></i></b><span style="font-size: 11.5pt;"><o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-size: 11.5pt; line-height: 115%;">FUNNELS,
FLUIDS, AND BBQ BACTERIA!<o:p></o:p></span></div>
<div class="Default">
<br /></div>
<div class="Default" style="margin-top: .15pt;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"> After thoroughly cleaning,
inspect your polysulfone or glass filter funnel for any deep nicks or
scratches. This can harbor bacteria and skew your count or ruin your sterile
control. <o:p></o:p></span></div>
<div class="Default" style="margin-top: .05pt;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Sterilize the funnel(s) initially in an autoclave, being sure not
to touch the inside walls of the funnel when handling. <o:p></o:p></span></div>
<div class="Default" style="margin-bottom: .0001pt; margin-bottom: 0in; margin-left: 0in; margin-right: 3.1pt; margin-top: 1.65pt; text-align: justify;">
<b><span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">TIP: Beware </span></b><span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">- <b>The funnels come out of the
autoclave HOT! </b><o:p></o:p></span></div>
<div class="Default" style="margin-right: 3.1pt; text-align: justify;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Let them cool, or dispense some
sterile dilution water down the interior walls of the funnel prior to filtering
the sample so as not to cause any flash thermal exposure (B-B-Q!) to the
bacteria, especially if filtering small sample volumes. <o:p></o:p></span></div>
<div class="MsoNormal">
Be sure to rinse the interior walls of the funnel after
sample filtration with sterile, and properly buffered dilution water. Typically
it will be slightly basic.<o:p></o:p></div>
<div class="Default">
<b><i><span style="font-size: 14.0pt;"><br /></span></i></b></div>
<div class="Default">
<b><i><span style="font-size: 14.0pt;"><br /></span></i></b></div>
<div class="Default">
<b><i><span style="font-size: 14.0pt;">THREE: </span></i></b><span style="font-size: 14.0pt;"><o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-size: 11.5pt; line-height: 115%;">INCUBATORS:
“THIS ONE’S TOO HOT, THIS ONE’S TOO COLD, THIS ONE’S JUST RIGHT!”<o:p></o:p></span></div>
<div class="Default">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Different bacteria
require specific temperatures for optimum growth. Total Coliform are fine at 35
degrees Celsius, while <b><i>E. coli </i></b>– a part of the Coliform Group are
thermotolerant and like it hot – at 44.5. degrees C. <o:p></o:p></span></div>
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">When incubating bacteria, because it’s your job to
grow them, be sure that the incubator is at the correct temperature not only
during the thermometer check times, but use a continuously monitoring </span><span style="font-family: Calibri, sans-serif; font-size: 11pt;">device,
(plugs into your laptop), so you know for sure that the incubator held
temperature during the whole incubation period.</span><br />
<div class="Default">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Use a
thermometer that has been calibrated against a NIST traceable thermometer. <o:p></o:p></span></div>
<div class="MsoNormal">
When laying your membrane filter on the prepared media
plated or other media source, place one portion of the filter at an angle onto
the media surface – then while applying a very slightly downward pressure
simply roll the rest of the filter onto the media surface using your sterile
forceps or clips.<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="Default">
<b><i><span style="font-size: 11.5pt;">FOUR: </span></i></b><span style="font-size: 11.5pt;"><o:p></o:p></span></div>
<div class="Default">
<b><span style="font-size: 10.0pt;">TIP: Do <i>at least 5 </i>serial
dilutions, more if you have the sample material. </span></b><span style="font-size: 10.0pt;"><o:p></o:p></span></div>
<div class="Default">
<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; mso-ascii-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Be sure to
incubate your cultures upside down – media side up, to keep the moisture level
of the media up. <o:p></o:p></span></div>
<div class="MsoNormal">
Following these simple rules will go a very long way in
assuring you get great results with your DMRQA test in membrane filtration!
Good Luck!<o:p></o:p></div>
<div class="MsoNormal">
“Membrane Filtration is used the world around – and for very
good reason<b><i>… It works!”<o:p></o:p></i></b></div>
<div class="MsoNormal">
<b><i><br /></i></b></div>
<div class="MsoNormal">
<b><i><span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">Check
out MIDSCI’s<a href="http://shop.midsci.com/scategory/M50/2457/Microbiology/Pre-poured_media_petri_dishes/"> pre-poured plates</a></span></i></b></div>
<div class="separator" style="clear: both; text-align: center;">
<b><i><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgD8-IvkqCaw8EyofJYUF5nvE05vMxN59ES4d_wPiUzrnGKDF_dCNH1qhr7VdTs5cDtmfJw-32Q3OVO0x1BfZL5sD6zwD-eDwrUkjlW0ovKAqtpdOaELfzRE9maxIsRWMN-J2m9-YDIC3un/s1600/prepoured+plate.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgD8-IvkqCaw8EyofJYUF5nvE05vMxN59ES4d_wPiUzrnGKDF_dCNH1qhr7VdTs5cDtmfJw-32Q3OVO0x1BfZL5sD6zwD-eDwrUkjlW0ovKAqtpdOaELfzRE9maxIsRWMN-J2m9-YDIC3un/s1600/prepoured+plate.jpg" height="270" width="320" /></a></i></b></div>
<b><i>. </i></b>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-41237936009738061812014-10-20T11:07:00.002-07:002014-10-20T11:07:50.393-07:00Improving your Western Blots!<div class="MsoNormal">
<b><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">MIDSCI now carries new Expedeon® RunBlue™ Pre-Cast gels!</span></b><o:p></o:p></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">Check out our <a href="http://shop.midsci.com/scategory/M50/2434/Electrophoresis_and_Blotting/Pre-Cast_Gels/">options</a>
to fit your unit today!<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><span style="color: blue;"><b>Seven tips to improving your Western Blots:</b></span><o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><span style="color: blue;"><b><br /></b></span></span></div>
<div class="MsoListParagraphCxSpFirst" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 1. </span><span style="background-color: white;"><span style="color: red;">Uniformly distributed high background?</span></span><o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->You may have insufficiently washed. Increase the number of washes and buffer
volume. <o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">§<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Make washing easier with <a href="http://shop.midsci.com/scategory/M50/2192/Electrophoresis_and_Blotting/Blot_Boxes/MIDSCI_Western_Blot_Boxes/">Blot
Boxes</a>.<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Antibody concentrations too high<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">§<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Make sure to optimize your antibody
concentrations by running strips at varying concentrations to determine best
concentration for your samples.<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 2. </span><!--[endif]--><span style="color: red;">Weak or no signal?</span><o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Your protein may have not transferred well. <o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">§<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Check your transfer buffer make-up and blotting
procedure. Stain gel to visualize
proteins remaining in gel. <o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 3. </span><!--[endif]--><span style="color: red;">The Ladder Lane is black</span><o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->The antibody may be reacting with your MW
(ladder) marker.<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">§<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Add an empty lane between the MW marker and
sample lane.<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 4. </span><!--[endif]--><span style="color: red;">The band of interest is too low/too high on the
blot.</span><o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Separation is not efficient. <o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">§<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Change the gel percentage: A higher percentage
for small protein, a lower percentage for larger proteins. Or try a <a href="http://shop.midsci.com/productdetail/M50/BCG42012/RunBlue_SDS_Gel_4-20%25_12_well_8x10_-_pack_of_10_fits_Bio-Rad_units/">gradient</a>
gel.<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.5in; mso-add-space: auto; mso-list: l0 level3 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 5. </span><!--[endif]--><span style="color: red;">Ripped resolving gel when moving to the cassette
for transferring?</span><o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Try Run Blue™ pre-cast gels from MIDSCI. They are stronger and resist tearing! No more
piecing together torn gels!<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 6. </span><!--[endif]--><span style="color: red;">Sample bleeding over to another well or
difficulty loading?</span></span><o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Try the deeper wells from Expedeon! Easily hold 35ul of sample without bleeding
over to another well!<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Try <a href="http://shop.midsci.com/scategory/M50/758/Liquid_Handling/Pipette_Tips/Gel_Loading_Tips/Avant_200ul_Gel_Loading_Tips/">gel-loading
tips</a>. Available in flat or round,
they make gel loading easier!<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;"><br /></span></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">·<span style="font-size: 7pt; font-stretch: normal;"> 6. </span><!--[endif]--><span style="color: red;">Worried about pre-cast gels expiring or losing
resolution before you can use them?</span><o:p></o:p></span></div>
<br />
<div class="MsoListParagraphCxSpLast" style="margin-left: 1.0in; mso-add-space: auto; mso-list: l0 level2 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Helvetica Neue, Arial, Helvetica, sans-serif;">o<span style="font-size: 7pt; font-stretch: normal;">
</span><!--[endif]-->Expedeon Run Blue™ gels have a 2 year shelf life
with no loss in resolution. Use them
confidently from purchase date to 2 years later!</span><o:p></o:p></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-1488473687327651392014-09-09T14:42:00.001-07:002014-09-09T14:42:47.341-07:00MIDSCI offers Quality and Affordability with new Saliva Kits!<div class="MsoNormal">
Did you know MIDSCI now carries Genefix Saliva
kits? <o:p></o:p></div>
<div class="MsoNormal">
Besides a more affordable,
yet high quality option, how do our Saliva kits compare to others on the
market? Check it out below and please
contact us with any questions!<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<!--[if gte vml 1]><v:shapetype
id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
<v:stroke joinstyle="miter"/>
<v:formulas>
<v:f eqn="if lineDrawn pixelLineWidth 0"/>
<v:f eqn="sum @0 1 0"/>
<v:f eqn="sum 0 0 @1"/>
<v:f eqn="prod @2 1 2"/>
<v:f eqn="prod @3 21600 pixelWidth"/>
<v:f eqn="prod @3 21600 pixelHeight"/>
<v:f eqn="sum @0 0 1"/>
<v:f eqn="prod @6 1 2"/>
<v:f eqn="prod @7 21600 pixelWidth"/>
<v:f eqn="sum @8 21600 0"/>
<v:f eqn="prod @7 21600 pixelHeight"/>
<v:f eqn="sum @10 21600 0"/>
</v:formulas>
<v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
<o:lock v:ext="edit" aspectratio="t"/>
</v:shapetype><v:shape id="Picture_x0020_2" o:spid="_x0000_i1025" type="#_x0000_t75"
style='width:375pt;height:526.5pt;visibility:visible;mso-wrap-style:square'>
<v:imagedata src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image001.png"
o:title=""/>
</v:shape><![endif]--><!--[if !vml]--><!--[endif]--><o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhXq1I6Iz5K_4mv3BUoaRahR2WRwUuftZFnNKmAStdVIX5vkfZJqRTXPFGgNdr8nP3nl0p6CronnNy68o_HYAkDi_p6OeHIlnQ7g0sheZBcdHED6H9E-eaojXPUrLjS6BN-o0ndDRhJmGYa/s1600/blog+post.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhXq1I6Iz5K_4mv3BUoaRahR2WRwUuftZFnNKmAStdVIX5vkfZJqRTXPFGgNdr8nP3nl0p6CronnNy68o_HYAkDi_p6OeHIlnQ7g0sheZBcdHED6H9E-eaojXPUrLjS6BN-o0ndDRhJmGYa/s1600/blog+post.jpg" height="640" width="456" /></a></div>
<br />
<div class="MsoNormal">
Genefix Saliva Collectors from MIDSCI contain the
stabilization buffer in the tube, allowing for rapid sample stabilization that
is maintained at room temperature for at least 12 months. The unique funnel design means no buffer flow
back or spillages and the easy to use design allows for use in the field, at
home, or clinic based sampling. The
collection tube, certified to 95 kPa, allows for leak-proof mailing. Saliva DNA Isolation kits are available in
both column based and buffer (no column based kits) allowing for both manual
and high throughput isolation applications.
<o:p></o:p></div>
<div class="MsoNormal">
Want to
see the results for yourself? Contact us
for a sample or to purchase a kit at <a href="mailto:custserv@midsci.com">custserv@midsci.com</a>. For any technical questions, please feel free
to e-mail <a href="mailto:elise.ambrose@midsci.com">elise.ambrose@midsci.com</a>. <o:p></o:p></div>
<br />
<div class="MsoNormal">
<br /></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-21374901206787044272014-06-23T12:11:00.001-07:002014-06-23T12:12:32.662-07:00Choosing a Latex or Nitrile Glove for your needs<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">Gloves are an essential part of
most laboratories. 4 criteria must be
met for high quality gloves:<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpFirst" style="margin-left: 76.8pt; mso-add-space: auto; mso-list: l2 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Excellent tactile sensitivity<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 76.8pt; mso-add-space: auto; mso-list: l2 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Provide barrier protection<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 76.8pt; mso-add-space: auto; mso-list: l2 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Remain non-reactive when in contact with patient
or sample<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpLast" style="margin-left: 76.8pt; mso-add-space: auto; mso-list: l2 level1 lfo1; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Provide comfort over extended wear times<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">MIDSCI™ carries high quality <a href="http://www.midsci.com/lp/Aurelia_Gloves_2014.html">nitrile and latex gloves</a>. With all of MIDSCI™’s extensive choices, you
will not only be able to find a glove that fits your needs, but it will be a
glove that you love!<o:p></o:p></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">When picking either latex or nitrile
gloves, it is important to know exactly which qualities are most important to
you. MIDSCI™ carries 4 main types of
latex gloves: <o:p></o:p></span></div>
<div class="MsoListParagraphCxSpFirst" style="mso-list: l3 level1 lfo2; text-indent: -.25in;">
</div>
<ul>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">·</span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; font-size: 7pt; text-indent: -0.25in;"> </span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">Luminance™</span></li>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">·</span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; font-size: 7pt; text-indent: -0.25in;"> </span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">Distinct™</span></li>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">·</span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; font-size: 7pt; text-indent: -0.25in;"> </span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">Velocity™</span></li>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">·</span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; font-size: 7pt; text-indent: -0.25in;"> </span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;">Vibrant™</span></li>
<ul>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;"> Sizes from extra-small to extra-large</span></li>
<li><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;"> All of the gloves are manufactured by <span style="text-indent: 0px;">Aurelia®</span> and undergo air testing for holes to ensure you receive high quality
gloves.</span><span style="color: blue; font-family: 'Trebuchet MS', sans-serif; text-indent: -0.25in;"> </span></li>
</ul>
</ul>
<br />
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Worried about tearing?</b> Distinct and Velocity are your best latex options
while maintaining great elasticity. The
Velocity™ is also Chlorine washed. The
Chlorination ensures the user can get the latex glove on easily without
powder. <o:p></o:p></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b><br /></b></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Worried about a latex allergy?</b> The Vibrant™ latex gloves are triple
washed to significantly reduce the risk of a latex allergy with super low
protein content. <o:p></o:p></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b><br /></b></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Need excellent grip?</b> The
polymer coating found in the Velocity™, Distinct™, and Luminance™ allows for
optimum comfort and increased gripping actions.
Many of the gloves are also textured to ensure maximum grip. <o:p></o:p></span></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<b><span style="color: blue; font-family: Trebuchet MS, sans-serif;">Are you instead looking
for Nitrile gloves?<o:p></o:p></span></b></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">MIDSCI™ carries Aurelia®’s extensive line of Nitrile
gloves. <o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">Aurelia®’s nitrile gloves offer:<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpFirst" style="margin-left: 38.4pt; mso-add-space: auto; mso-list: l4 level1 lfo4; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Superior comfort and durability<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 38.4pt; mso-add-space: auto; mso-list: l4 level1 lfo4; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Finger texture<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 38.4pt; mso-add-space: auto; mso-list: l4 level1 lfo4; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Air tested to detect pinholes<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpLast" style="margin-left: 38.4pt; mso-add-space: auto; mso-list: l4 level1 lfo4; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Extensive quality control check to ensure
optimum product<o:p></o:p></span></div>
<div class="MsoNormal" style="text-indent: 20.4pt;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">Nitrile gloves are offered from
the thinnest nitrile available on the market (Amazing™) to a thicker nitrile
glove for superior protection (Robust™).
<o:p></o:p></span></div>
<div class="MsoNormal" style="text-indent: 20.4pt;">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">MIDSCI™ carries 6 main types of
nitrile gloves:<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpFirst" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Amazing<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Perform<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Transform<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Quest<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpMiddle" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Protégé<o:p></o:p></span></div>
<div class="MsoListParagraphCxSpLast" style="margin-left: 56.4pt; mso-add-space: auto; mso-list: l0 level1 lfo5; text-indent: -.25in;">
<!--[if !supportLists]--><span style="color: blue; font-family: Trebuchet MS, sans-serif;">·<span style="font-size: 7pt;">
</span><!--[endif]-->Robust<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"> </span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Are you looking for high tactile sensitivity?</b> We would recommend any of the thin nitrile
gloves for this. Amazing™ gloves are the
thinnest, while Perform™ gloves offer strength comfort and sensitivity.<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b><br /></b></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Are you looking for
high tactile sensitivity while maintaining durability?</b> Transform™ gloves are thin yet durable.<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b><br /></b></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Are you looking for a
glove similar to latex in comfort, fit and cost, but need nitrile durability
and latex-free?</b> Try the Aurelia®
Quest™ glove! Quest™ gloves also maintain great chemical resistance and are
finger textured for optimum gripping.
The Protégé™ glove also rivals latex in comfort and ergonomics and comes
in a powder blue color.<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b><br /></b></span></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;"><b>Need a thicker
nitrile glove for improved puncture resistance?</b> The Robust™ offers a 5 mil thickness and
micro-textured finish for improved gripping action. <o:p></o:p></span></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<span style="color: blue; font-family: Trebuchet MS, sans-serif;">MIDSCI™ has a <a href="http://www.midsci.com/lp/Aurelia_Gloves_2014.html">glove</a> to fit your needs. Contact us today at 1-800-227-9997 for a
quote! For a limited time only, we are
offering Buy 15 get 5 free (of the same family)!<o:p></o:p></span></div>
<div class="MsoNormal">
<br /></div>
<br />
<div class="MsoNormal">
<br /></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-61277083631352622722014-04-21T09:44:00.000-07:002014-04-21T09:44:27.331-07:00PCR plates and strip tubes designed to reduce evaporation and save you money!<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> QPCR and PCR are common research focuses in many labs. Many of us have experienced inconsistencies
between samples or even triplicates from run to run or well to well. Did you know that evaporation may be to
blame? A small change in the volume of
buffers can alter the pH of the mix in the well, impacting the ability of the
enzyme to function efficiently. For
instance, at 74C for every 5ul evaporation loss of a 25ul reaction, the pH
changes by 1. What causes this
evaporation and how can we minimize it to result in more consistent, repeatable
experiments? Can we minimize evaporation
so much that we can even downscale our reactions?<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> A
standard one-piece plate with a polypropylene frame expands by up to 2mm during
thermal cycling (Figure 1). The issues
arise when the sealing film or strip caps expand and contract at different
rates, leaving a small space and opening up the well to evaporation. In addition, PCR blocks do not support PCR
plates from the side, allowing for the high temperatures of the thermal block
and heated lid to accelerate the expansion of the plates (Figure 2).<o:p></o:p></span></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh5m2lCRVPt7NKZvrefiszVNpVOa-CV-nekSqg1AN2JMTJBmaWNb6cBqAMpX33lGpkcf2NTyioCTu3be54jQEwztNZ1mW2Yf1smWFi584tziDHcvVVVKGf1iVNlScoaRRcQoqogGXvw0T4_/s1600/Figure+1+4titude+blog+post.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh5m2lCRVPt7NKZvrefiszVNpVOa-CV-nekSqg1AN2JMTJBmaWNb6cBqAMpX33lGpkcf2NTyioCTu3be54jQEwztNZ1mW2Yf1smWFi584tziDHcvVVVKGf1iVNlScoaRRcQoqogGXvw0T4_/s1600/Figure+1+4titude+blog+post.png" height="200" width="320" /></a></div>
<br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjvUZpxgBET8go8_hK80VoWudgi9Si52LPcyBAnS_MjQjcYK40bCUj01FZQZffpe3tdwtUs3esENarAJqf_Q5mz5HQjLU3o_UnzFWJ_k2czP6OJhhJpatbezJxufLAtfC-sFUWsBuBNqYP8/s1600/Figure+2+4titude+blog+post.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjvUZpxgBET8go8_hK80VoWudgi9Si52LPcyBAnS_MjQjcYK40bCUj01FZQZffpe3tdwtUs3esENarAJqf_Q5mz5HQjLU3o_UnzFWJ_k2czP6OJhhJpatbezJxufLAtfC-sFUWsBuBNqYP8/s1600/Figure+2+4titude+blog+post.png" height="193" width="320" /></a></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"><br /></span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: Trebuchet MS, sans-serif;">Evaporation in itself is an issue,
but a large issue also arises when uneven evaporation is considered. Generally evaporation occurs in the outside
wells (Figure 3). More evaporation in
one well over another means wells may have different pHs and thus differences
in enzyme efficiency. This becomes an
issue when comparing sample to sample or when trying to repeat experiments.</span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: Trebuchet MS, sans-serif;"><br /></span></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgHgf9lXR_vfBMq3DmPLibKF36NGocX8GmqzzrHv_m6IaWNk4b6mE-YL3zEVwsvVdG7mGVjhj9yrUQ-nYg4qB0-2YQraC82hyphenhyphenpviD98hTsc-VFBeU9U74rs1MUAiS7d-dHkqsivRwgf5gNQ/s1600/Figure+3+4titude+blog+post2.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgHgf9lXR_vfBMq3DmPLibKF36NGocX8GmqzzrHv_m6IaWNk4b6mE-YL3zEVwsvVdG7mGVjhj9yrUQ-nYg4qB0-2YQraC82hyphenhyphenpviD98hTsc-VFBeU9U74rs1MUAiS7d-dHkqsivRwgf5gNQ/s1600/Figure+3+4titude+blog+post2.jpg" height="157" width="200" /></a></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: Trebuchet MS, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> Two-piece
construction plates combat the issues of evaporation. In two-piece construction plates, the tubes
are made of polypropylene and the frame is polycarbonate. Polycarbonate is a more rigid plastic that
decreases the amount of thermal expansion, resulting in reduced evaporation and
this improved PCR performance. Two-piece
construction plates, due to the lower evaporation, allow for downscaling of PCR
reactions. <o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> In
comparing the volume loss of a one-piece plate to a two-piece construction
plate, the researcher is losing money as each microliter of enzyme
evaporates. For instance, as seen in
Table 1, the outer wells of a one-piece construction plate lose almost 3 µl
while the inner wells lose over 1.5 µl due to evaporation. 4titude®’s Framestar™ two-piece construction plates,
on the other hand, only lost 0.57 µl per well in the outer wells and a little
over 0.5ul in the inner wells. A loss of
more than 6 times in the one-piece plates, adds up significantly in money
loss. If you were to assume the master
mix costs about an average of 10 cents per microliter, then in the One-piece
plates, a researcher loses $23.32 per each 96 well plate due to evaporation
(Table 1). In contrast, a researcher would only lose
$5.33 in 4titude®’s Framestar™ two-piece construction plates. In PCR plates, small amounts of evaporation
per well result in not only reproducibility issues, but also in money
loss. Two-piece construction plates
greatly reduce those issues.<o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"><br /></span></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh-kE9WWzsEhJvjiSHYkbebXCH-CydGBvRmkQxSbvz-XYY6M_sOowUtejyWFuYPrv9kSCcKPdyzEINi6t0SdS2NhP41ZQUI-ORXalYt8tGSE9levsq3sKA8IOb8U9lpQx5A_4eJS5Sa_StI/s1600/Table+1+4titude+blog+post.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh-kE9WWzsEhJvjiSHYkbebXCH-CydGBvRmkQxSbvz-XYY6M_sOowUtejyWFuYPrv9kSCcKPdyzEINi6t0SdS2NhP41ZQUI-ORXalYt8tGSE9levsq3sKA8IOb8U9lpQx5A_4eJS5Sa_StI/s1600/Table+1+4titude+blog+post.png" height="308" width="640" /></a></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"><br /></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> </span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> 4titude's
lower evaporation per well, a researcher can save money by reducing the
reaction reagent volumes without compromising results. Dr. Carolyn Denier in Berlin was able to
reduce Sybr green usage by 40%, saving over $30 per plate and still maintain
the consistent CTs she had in the past.
384 well users also see great results with 4titude® plates. Dr. Andreas Dahl in Berlin was able to
downscale his reactions as well due to decreased evaporation. By reducing reaction volumes, a researcher
can save significantly over time. <o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;"> Overall,
4titude®’s Framestar™ plates and strip tubes have less thermal expansion for
improved sealing integrity resulting in lower sample evaporation. The lower sample evaporation allows customers
to maintain more consistent results and downscale their reactions to save
money. Due to the lower thermal expansion
of the 4titude® PCR plastics from MIDSCI, these plates are great for robotic
use in that they are more reliable for pipetting and stacking. The lower sample evaporation of 4titude’s PCR
plastics makes these plates and strip tubes great for low volume PCR. The plates also come in white well format for
higher sensitivity and even greater consistency from well to well. <o:p></o:p></span></div>
<div class="MsoNormal">
<span style="font-family: Trebuchet MS, sans-serif;">Call MIDSCI today to purchase the 4titude® cost saving
products!<o:p></o:p></span></div>
<br />
<div class="MsoNormal">
<br /></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-88289253257164873722014-02-06T09:10:00.000-08:002014-02-06T09:10:18.478-08:00<div class="MsoNormal">
<b>Improve DNA
collection, storage and purity with Isohelix Buccal Swabs and kits </b><o:p></o:p></div>
<div class="MsoNormal">
Buccal
swabs are used across a wide variety of fields.
They are used in research laboratories, forensic labs, genetic testing
laboratories, veterinary genotyping and diagnostics laboratories, paternity
testing laboratories, and clinical diagnostics, to name a few. The type of buccal swab used can not only
have an effect on the amount of DNA collected, but can also impact further
stability and extraction in downstream applications. Isohelix swabs have a novel matrix that
ensures efficient collection and increases DNA yield and ease of
extraction. When paired with Isohelix
kits, even higher yields and purity can be obtained.<o:p></o:p></div>
<div class="MsoNormal" style="text-indent: .5in;">
Isohelix provides an integrated
solution for DNA processing with products for sampling, transporting,
stabilizing, and extraction while maintaining high yields and purity. Isohelix swabs provide significant advantages
over other commercially available swabs. (Figure below)<o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicXxGl1WgiRPBcL9x9FWcl_LVFxQ7m7ZPHu0J2Fmdq9ovEvhMY2gWA5Us75Idoyh743wHHauNekBX-Mnb-9C3wzehD4Wx03HOF2ZEsBt3TwIk_wiaXDCsnBg5R50psOVCbL7N6ujTmGPSH/s1600/isohelix+blog+post+1.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicXxGl1WgiRPBcL9x9FWcl_LVFxQ7m7ZPHu0J2Fmdq9ovEvhMY2gWA5Us75Idoyh743wHHauNekBX-Mnb-9C3wzehD4Wx03HOF2ZEsBt3TwIk_wiaXDCsnBg5R50psOVCbL7N6ujTmGPSH/s1600/isohelix+blog+post+1.png" height="241" width="400" /></a></div>
<br /><div class="MsoNormal">
What sets Isohelix apart?<o:p></o:p></div>
<div class="MsoNormal" style="margin-left: .5in; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: "Arial","sans-serif"; mso-fareast-font-family: Arial;">•<span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><!--[endif]--><b>High
yielding swabs for buccal cell DNA collection</b><o:p></o:p></div>
<div class="MsoNormal" style="margin-left: .5in; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: "Arial","sans-serif"; mso-fareast-font-family: Arial;">•<span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><!--[endif]--><b>Unique
swab matrix greatly improves DNA yields</b><o:p></o:p></div>
<div class="MsoNormal" style="margin-left: .5in; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: "Arial","sans-serif"; mso-fareast-font-family: Arial;">•<span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><!--[endif]--><b>Alternative
to blood collection</b><o:p></o:p></div>
<div class="MsoNormal" style="margin-left: .5in; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: "Arial","sans-serif"; mso-fareast-font-family: Arial;">•<span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><!--[endif]--><b>Suitable
for human and veterinary use</b><o:p></o:p></div>
<div class="MsoNormal" style="text-indent: .25in;">
There are various different swab
types available to fit your needs. All
swabs are ethylene oxide treated and are tested for DNA contamination. The SK-1 swabs come with a 5 mL tube. After
collecting the shaft can be easily snapped above swab head. The SK-1 swabs are best paired with
Dri-capsules, which allow for storage of the DNA for at least 3.5 years at room
temperature! <o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhk1NSx1uCC_PQvi-zzu5RAYA_SFbcsEJmqEO8Kl9pj8qXtr0BLLerQjhckkXSf4urqKxuVWz7ceBwE_0PCw1LqReNC2bSed6rW-YY4Bi_jU7vr5w-yQDljedBpOE0YY1LO05oJqjyfzClZ/s1600/sgc-50.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhk1NSx1uCC_PQvi-zzu5RAYA_SFbcsEJmqEO8Kl9pj8qXtr0BLLerQjhckkXSf4urqKxuVWz7ceBwE_0PCw1LqReNC2bSed6rW-YY4Bi_jU7vr5w-yQDljedBpOE0YY1LO05oJqjyfzClZ/s1600/sgc-50.jpg" height="170" width="400" /></a></div>
<div class="MsoNormal" style="text-indent: .25in;">
<!--[if gte vml 1]><v:shape
id="_x0000_i1027" type="#_x0000_t75" style='width:255pt;height:172.5pt;
visibility:visible;mso-wrap-style:square'>
<v:imagedata src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image003.png"
o:title=""/>
</v:shape><![endif]--><!--[if !vml]--><br /><o:p></o:p></div>
<div class="MsoNormal" style="text-indent: .25in;">
<span style="text-indent: 0.25in;">The swabs can also be purchased with a 2 mL
tube.</span><span style="text-indent: 0.25in;"> </span><span style="text-indent: 0.25in;">After collection, the lid on the 2
mL tube is securely closed.</span><span style="text-indent: 0.25in;"> </span><span style="text-indent: 0.25in;">If air
venting is desired for drying of the swab in the field, the stopper can be
opened while still having a closed cap.</span><span style="text-indent: 0.25in;">
</span><span style="text-indent: 0.25in;">The swab can then be removed by pulling on the shaft.</span><span style="text-indent: 0.25in;"> </span></div>
<div class="MsoNormal" style="text-indent: .25in;">
<o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgVY2GnygNXWOc-sQYlkhV95W82NmxJ3F_neuyv2rbMwwI_ghOgWSo_gNUOVFx0Vxg3zlm3T6gU0Z2KNAOGUidaXfIUkB0cFeA_7CqDdEaLaH3ilugdYepEOSBIkbROkX58Jl1oOpLsfpj8/s1600/isohelix+blog+post+3.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgVY2GnygNXWOc-sQYlkhV95W82NmxJ3F_neuyv2rbMwwI_ghOgWSo_gNUOVFx0Vxg3zlm3T6gU0Z2KNAOGUidaXfIUkB0cFeA_7CqDdEaLaH3ilugdYepEOSBIkbROkX58Jl1oOpLsfpj8/s1600/isohelix+blog+post+3.png" height="320" width="286" /></a></div>
<br /><div class="MsoNormal">
If desired, swabs can also be purchased individually
wrapped, two per pack, or three per pack.
<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<i>Need to effectively
stabilize and/or isolate your DNA?<o:p></o:p></i></div>
<div class="MsoNormal">
The DDK kit is a no column quick processing kit. The DDK kit includes a stabilization reagent
(stabilizes for up to 5 years) and isolation reagent that maintains both high
yield and purity. Up to 10 µg of DNA can
be obtained from each swab with the DDK kit.
<o:p></o:p></div>
<div class="MsoNormal">
The two cartridge based kits, XtraClean and Xtreme are
important when extra high purity is desirable.
These kits are great when downstream analysis assays are arrays or
quantitative PCR. <br />
<!--[if !supportLineBreakNewLine]--><br />
<!--[endif]--><o:p></o:p></div>
<div class="MsoNormal">
<i>Need your DNA Rapidly?<o:p></o:p></i></div>
<div class="MsoNormal">
Try the BEK Buccalyse DNA Release Kit. Obtain a 2 to 4µg yield in minutes! <o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_9cDIlD6RMouFpYOQJwlygHJ-0n_Tya_CtP79fmAjRT645VTCvYl94asi3Llb_R-epDqjxH7JaeMOdHscyJcoSbI1RhA86EoTwWA0Y5ysXUcH17SXScwfvxLkg-lkj0k7jmVmmYcfxLiH/s1600/isohelix+blog+post+4.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_9cDIlD6RMouFpYOQJwlygHJ-0n_Tya_CtP79fmAjRT645VTCvYl94asi3Llb_R-epDqjxH7JaeMOdHscyJcoSbI1RhA86EoTwWA0Y5ysXUcH17SXScwfvxLkg-lkj0k7jmVmmYcfxLiH/s1600/isohelix+blog+post+4.jpg" height="212" width="640" /></a></div>
<a href="http://shop.midsci.com/scategory/M50/2299/Clinical/Isohelix_Buccal_Swabs,_Stabalization_and_DNA_Isolation/">Isohelix swabs and kits</a> can increase not only your DNA yield, but
stability, purity, and ease of extraction.
Contact MIDSCI today for more information. <div class="MsoNormal">
<o:p></o:p></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-7094789558757670732014-01-03T18:21:00.001-08:002014-01-03T18:21:41.791-08:00Bioreactors: What are they and how can they improve experiments?<div class="separator" style="clear: both; text-align: center;">
</div>
<br />
<div class="separator" style="clear: both; text-align: center;">
</div>
<div class="MsoNormal">
Have you ever had to clean out Erlenmeyer flasks or glass
bottles after growing bacteria or suspension cells? Did you ever forget to
autoclave bottles and then had to wait to start your experiment? Have you ever
wanted to grow and spin suspension cells in the same vessel? Would you like to vary gas exchange without
opening up the culture to contamination?<o:p></o:p></div>
<div class="MsoNormal">
Disposable bioreactors are the solution to many of these
common issues in growing suspension and microbial cultures. <o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<b><span style="color: #1f497d; mso-themecolor: text2;">What is a bioreactor? <o:p></o:p></span></b></div>
<div class="MsoNormal">
Bioreactors are growth vessels (of varying types) that
provide similar cell physiology to in vivo allowing for optimal cell growth and
high level productivity.<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<b><span style="color: #1f497d; mso-themecolor: text2;">How do bioreactors accomplish this?<o:p></o:p></span></b></div>
<div class="MsoNormal">
Bioreactors optimize cell growth and lead to high level
productivity by maintaining the following characteristics:<o:p></o:p></div>
<div class="MsoListParagraphCxSpFirst" style="mso-list: l1 level1 lfo1; text-indent: -.25in;">
</div>
<ul>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Stable temperature</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Maintenance of oxygen and gas level</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Physiological pH</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Low shear stress</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Rapid response to induced changes</span></li>
</ul>
<!--[if !supportLists]--><o:p></o:p><br />
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l1 level1 lfo1; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l1 level1 lfo1; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l1 level1 lfo1; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpLast" style="mso-list: l1 level1 lfo1; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoNormal">
<b><span style="color: #1f497d; mso-themecolor: text2;">How to choose a bioreactor for your
needs—(what are important qualities of a bioreactor?):</span></b><o:p></o:p></div>
<div class="MsoListParagraphCxSpFirst" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Symbol; mso-bidi-font-family: Symbol; mso-fareast-font-family: Symbol;">·<span style="font-family: 'Times New Roman'; font-size: 7pt;">
</span></span></div>
<!--[endif]--><!--[if gte vml 1]><v:shapetype id="_x0000_t75"
coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe"
filled="f" stroked="f">
<v:stroke joinstyle="miter"/>
<v:formulas>
<v:f eqn="if lineDrawn pixelLineWidth 0"/>
<v:f eqn="sum @0 1 0"/>
<v:f eqn="sum 0 0 @1"/>
<v:f eqn="prod @2 1 2"/>
<v:f eqn="prod @3 21600 pixelWidth"/>
<v:f eqn="prod @3 21600 pixelHeight"/>
<v:f eqn="sum @0 0 1"/>
<v:f eqn="prod @6 1 2"/>
<v:f eqn="prod @7 21600 pixelWidth"/>
<v:f eqn="sum @8 21600 0"/>
<v:f eqn="prod @7 21600 pixelHeight"/>
<v:f eqn="sum @10 21600 0"/>
</v:formulas>
<v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
<o:lock v:ext="edit" aspectratio="t"/>
</v:shapetype><v:shape id="Picture_x0020_2" o:spid="_x0000_s1029" type="#_x0000_t75"
alt="Picture1.png" style='position:absolute;left:0;text-align:left;
margin-left:271.05pt;margin-top:1.65pt;width:158.35pt;height:107.05pt;
z-index:251662336;visibility:visible;mso-wrap-style:square;
mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;
mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;
mso-position-horizontal-relative:text;mso-position-vertical:absolute;
mso-position-vertical-relative:text'>
<v:imagedata src="file:///C:\DOCUME~1\ELISEA~1\LOCALS~1\Temp\msohtmlclip1\01\clip_image001.png"
o:title="Picture1"/>
<w:wrap type="square"/>
</v:shape><![endif]--><!--[if !vml]--><!--[endif]--><div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhOrXpngimECY2DDXPYu1O2j52Aa9dSMiBomuuxvYQxgUpgR7FlgELY0ArYSNh33wzeP3iOaZy0s9d_OwhCVFIcpSjHFeqvaaMquBvps238Xjfu2PB9rAP6WQ6TdgffXfMugPIkpk3EEQv4/s1600/pic1.png" imageanchor="1" style="clear: right; float: right; margin-bottom: 1em; margin-left: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhOrXpngimECY2DDXPYu1O2j52Aa9dSMiBomuuxvYQxgUpgR7FlgELY0ArYSNh33wzeP3iOaZy0s9d_OwhCVFIcpSjHFeqvaaMquBvps238Xjfu2PB9rAP6WQ6TdgffXfMugPIkpk3EEQv4/s1600/pic1.png" height="212" width="320" /></a></div>
<ul>
<li><span style="text-indent: -0.25in;">Ease
of use</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Rapid set up</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Disposable</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Efficient gas exchange and even dispersing of
nutrients (Figure 1)</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Low shear stress</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Minimal impact of errors and contamination</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">Lower chance of contamination</span></li>
</ul>
<div style="text-indent: -24px;">
<span style="text-indent: 0px;"> MIDSCI is the United States’ exclusive importer of TPP bioreactors (Figure 2). </span></div>
<o:p></o:p><br />
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpLast" style="mso-list: l0 level1 lfo2; text-indent: -.25in;">
<o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-J3SpaD4xehzOnwtHxiE8bBYL8nm3N95FAJ8Qd61o2sk_bgjMGPmQdVwF5kPSKkquT-pV000V8Ie9N0WN4GsGc6m0ZgG49dCE2SR60irUxTKHzzIXOYFaGxG4x0B4bOsUq4J383IYRil1/s1600/pic2.png" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-J3SpaD4xehzOnwtHxiE8bBYL8nm3N95FAJ8Qd61o2sk_bgjMGPmQdVwF5kPSKkquT-pV000V8Ie9N0WN4GsGc6m0ZgG49dCE2SR60irUxTKHzzIXOYFaGxG4x0B4bOsUq4J383IYRil1/s1600/pic2.png" height="113" width="200" /></a></div>
<div class="MsoNormal">
<!--[if gte vml 1]><v:shape id="Picture_x0020_4" o:spid="_x0000_s1028"
type="#_x0000_t75" alt="Picture2.png" style='position:absolute;margin-left:-8.55pt;
margin-top:14.9pt;width:141.2pt;height:80.1pt;z-index:251664384;visibility:visible;
mso-wrap-style:square;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
mso-position-vertical:absolute;mso-position-vertical-relative:text'>
<v:imagedata src="file:///C:\DOCUME~1\ELISEA~1\LOCALS~1\Temp\msohtmlclip1\01\clip_image003.png"
o:title="Picture2"/>
<w:wrap type="square"/>
</v:shape><![endif]--><!--[if !vml]--><!--[endif]--><o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<br /><div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<b><span style="color: #1f497d; mso-themecolor: text2;">What sets TPP bioreactors apart?<o:p></o:p></span></b></div>
<div class="MsoListParagraph" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
</div>
<ul>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">TPP bioreactors have more efficient shaking (due
to the internal cyclonic stirring) compared to the Erlenmeyer flask and bottle
growth vessel (Figure3)</span></li>
</ul>
<!--[if !supportLists]--><o:p></o:p><br />
<div class="MsoNormal" style="margin-left: .25in;">
<!--[if gte vml 1]><v:shape
id="Picture_x0020_5" o:spid="_x0000_i1025" type="#_x0000_t75" alt="Picture3.png"
style='width:340.5pt;height:165.75pt;visibility:visible;mso-wrap-style:square'>
<v:imagedata src="file:///C:\DOCUME~1\ELISEA~1\LOCALS~1\Temp\msohtmlclip1\01\clip_image005.png"
o:title="Picture3"/>
</v:shape><![endif]--><!--[if !vml]--><!--[endif]--><o:p></o:p></div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgHA5nVTfZmPvPSNjVta3nnOBXWsQaiGqnrCecg0lXv3zePxbhl2uH8H5u-xWuNy2089d3u0eG25O89lbh9CUj6wr33JmgT5qq6hnlJleIseIxo48jLLIgWb1NHjVe430mjyy979hwlIVCp/s1600/pic3.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgHA5nVTfZmPvPSNjVta3nnOBXWsQaiGqnrCecg0lXv3zePxbhl2uH8H5u-xWuNy2089d3u0eG25O89lbh9CUj6wr33JmgT5qq6hnlJleIseIxo48jLLIgWb1NHjVe430mjyy979hwlIVCp/s1600/pic3.png" height="361" width="640" /></a></div>
<div class="MsoListParagraphCxSpFirst" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
</div>
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgIzBPrbm0uMzdEXWMW2s9-9TRHb_rvgNeNyMt8m9UmH4hEGOgOQts1PBOl002-1QqSHKCP3nrhHbXA7Cejx3zFkuN2rC-hrFs33muAkgIAH45BzxiOy7-N0lXiRx_cYS7rK4LZMlVSKrmO/s1600/pic4.png" imageanchor="1" style="clear: right; float: right; margin-bottom: 1em; margin-left: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgIzBPrbm0uMzdEXWMW2s9-9TRHb_rvgNeNyMt8m9UmH4hEGOgOQts1PBOl002-1QqSHKCP3nrhHbXA7Cejx3zFkuN2rC-hrFs33muAkgIAH45BzxiOy7-N0lXiRx_cYS7rK4LZMlVSKrmO/s1600/pic4.png" height="200" style="cursor: move;" width="170" /></a></div>
<ul>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">TPP
bioreactors have no excess CO</span><sub style="text-indent: -0.25in;">2 </sub><span style="text-indent: -0.25in;">as the efficient shaking allows for
proper gas exchange and dispersal of nutrients (Figure 1)</span></li>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">TPP bioreactors have no excess foam or shear
stress (Figure 4)</span></li>
</ul>
<!--[if !supportLists]--><o:p></o:p><br />
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
</div>
<ul>
<li><span style="font-family: Symbol; text-indent: -0.25in;"><span style="font-family: 'Times New Roman'; font-size: 7pt;"> </span></span><span style="text-indent: -0.25in;">TPP bioreactors allow for customizable gas
exchange with their 5 cap openings.
(Figure 5)</span></li>
</ul>
<!--[if !supportLists]--><o:p></o:p><br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEihA5TU9KB6JRtJqcAeP0nUsyzSCa7uUqzCrG_UAsS_wos68JzoQkEV5xIf0_BOKKSfbt_UmmF5E9HVxZ1Um5u89SVoNlnb_n1evvA_X_yAVPFIwsxljo8Gsfb0_mjM5yN4zMdzQEdlq-G3/s1600/pic5.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEihA5TU9KB6JRtJqcAeP0nUsyzSCa7uUqzCrG_UAsS_wos68JzoQkEV5xIf0_BOKKSfbt_UmmF5E9HVxZ1Um5u89SVoNlnb_n1evvA_X_yAVPFIwsxljo8Gsfb0_mjM5yN4zMdzQEdlq-G3/s1600/pic5.png" height="139" style="cursor: move;" width="200" /></a></div>
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
<!--[if !supportLists]--><span style="font-family: Symbol; mso-bidi-font-family: Symbol; mso-fareast-font-family: Symbol;">·<span style="font-family: 'Times New Roman'; font-size: 7pt;">
</span></span></div>
<!--[endif]--><!--[if gte vml 1]><v:shape id="_x0000_s1026"
type="#_x0000_t75" alt="Picture5.png" style='position:absolute;left:0;
text-align:left;margin-left:10.25pt;margin-top:4.7pt;width:103.65pt;height:1in;
z-index:251665408;visibility:visible;mso-wrap-style:square;
mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;
mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;
mso-position-horizontal-relative:text;mso-position-vertical:absolute;
mso-position-vertical-relative:text'>
<v:imagedata src="file:///C:\DOCUME~1\ELISEA~1\LOCALS~1\Temp\msohtmlclip1\01\clip_image009.png"
o:title="Picture5"/>
<w:wrap type="square"/>
</v:shape><![endif]--><!--[if !vml]--><!--[endif]--><ul>
<li>TPP
bioreactors can be <a href="http://shop.midsci.com/scategory/M50/2321/Equipment/Shakers/Scilogex_Shaker/">shaken</a>
and grown in a normal <a href="http://shop.midsci.com/scategory/M50/351/Equipment/Incubators/CO2_Incubators/Water_Jacketed_CO2_Incubators/">CO<sub>2</sub>
incubator</a>.</li>
<li><span style="text-indent: -0.25in;">15 and 50 mL TPP bioreactors can easily be spun
down in a </span><a href="http://shop.midsci.com/productdetail/M50/420CELLCULTURE4/Hettich_Rotina420_Centrifuge,_Cell_Culture,_Package-4,_Ambient/" style="text-indent: -0.25in;">centrifuge</a><span style="text-indent: -0.25in;">
without transferring. Rotors for the 600
mL TPP bioreactor are soon to come. The
less steps, the less of a chance there is of the introduction of
contaminants. </span></li>
<li><span style="text-indent: -0.25in;">Can easily scale up or scale down experiments.</span></li>
</ul>
<o:p></o:p><br />
<div class="MsoListParagraphCxSpMiddle" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoListParagraphCxSpLast" style="mso-list: l2 level1 lfo3; text-indent: -.25in;">
<o:p></o:p></div>
<div class="MsoNormal" style="margin-left: .25in;">
<br /></div>
<div class="MsoNormal">
MIDSCI has 15, 50, and 600 mL disposable <a href="http://shop.midsci.com/scategory/M50/2105/Tissue_Culture/TPP_Tissue_Culture_Plastics/TPP_TubeSpin_Bioreactors/">bioreactors</a>
available in various packaging sizes.
The 15 mL is available in 200, 400, and 800 items/case. The 50 mL is available in 60 and 180
items/case. The 600 mL is available in
3, 13, and 26 items/case. <o:p></o:p></div>
<div class="MsoNormal">
Have any questions?
Interested in learning more about our bioreactors? Contact MIDSCI today! <o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<br /></div>
<br />
<div class="MsoNormal">
<br /></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-5802373043982375702013-10-21T06:33:00.001-07:002013-10-21T06:33:22.860-07:00TPP tissue culture products help prevent contamination!
<br />
<div class="WordSection1">
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<span style="font-family: Calibri;"><b style="mso-bidi-font-weight: normal;">Problem</b>: Tissue
culture is a vital element of many research laboratories.<span style="mso-spacerun: yes;"> </span>Extensive experiments often stem off of
cultured cells and provide valuable insight.<span style="mso-spacerun: yes;">
</span>Any break in the ability to use cultured cells or newly isolated primary
cells impacts many experiments down the line.<span style="mso-spacerun: yes;">
</span>Growth of unwanted microbes and mold in tissue culture impairs the
ability of a researcher to perform further experiments.<span style="mso-spacerun: yes;"> </span>The researcher must then use valuable time
and resources to obtain new cells and avoid contamination in the future.<span style="mso-spacerun: yes;"> </span>Contamination in tissue culture is a complex
and frustrating issue afflicting thousands of researchers every year.<span style="mso-spacerun: yes;"> </span>Contamination is not only costly, but
results in great productivity loss.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<span style="font-family: Calibri;"><b style="mso-bidi-font-weight: normal;">Solution:<span style="mso-spacerun: yes;"> </span></b>TPP, distributed in the United States by
MIDSCI, addresses each contributing issue to contamination with its innovative
and unique design.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<span style="font-family: Calibri;"><span style="mso-tab-count: 1;"> </span><o:p></o:p></span></div>
</div>
<span style="font-family: "Calibri","sans-serif"; font-size: 11pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"><br clear="all" style="mso-break-type: section-break; page-break-before: always;" />
</span>
<br />
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<v:shapetype coordsize="21600,21600" filled="f" id="_x0000_t75" o:preferrelative="t" o:spt="75" path="m@4@5l@4@11@9@11@9@5xe" stroked="f"><span style="font-family: Calibri;">
<v:stroke joinstyle="miter">
<v:formulas>
<v:f eqn="if lineDrawn pixelLineWidth 0">
<v:f eqn="sum @0 1 0">
<v:f eqn="sum 0 0 @1">
<v:f eqn="prod @2 1 2">
<v:f eqn="prod @3 21600 pixelWidth">
<v:f eqn="prod @3 21600 pixelHeight">
<v:f eqn="sum @0 0 1">
<v:f eqn="prod @6 1 2">
<v:f eqn="prod @7 21600 pixelWidth">
<v:f eqn="sum @8 21600 0">
<v:f eqn="prod @7 21600 pixelHeight">
<v:f eqn="sum @10 21600 0">
</v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:f></v:formulas>
<v:path gradientshapeok="t" o:connecttype="rect" o:extrusionok="f">
<o:lock aspectratio="t" v:ext="edit">
</o:lock></v:path></v:stroke></span></v:shapetype><v:shape id="Picture_x0020_2" o:spid="_x0000_s1029" style="height: 120pt; margin-left: 154.5pt; margin-top: 249.75pt; mso-position-horizontal-relative: margin; mso-position-horizontal: absolute; mso-position-vertical-relative: margin; mso-position-vertical: absolute; mso-wrap-distance-bottom: 0; mso-wrap-distance-left: 9pt; mso-wrap-distance-right: 9pt; mso-wrap-distance-top: 0; mso-wrap-style: square; position: absolute; visibility: visible; width: 139.2pt; z-index: 251663360;" type="#_x0000_t75"><span style="font-family: Calibri;">
<v:imagedata o:title="" src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image001.png">
<w:wrap anchorx="margin" anchory="margin" type="square">
</w:wrap></v:imagedata></span></v:shape><span style="font-family: Calibri;">One of the biggest
obstacles to overcoming and avoiding contamination is removing the optimal
breeding grounds for microbes and mold spores.<span style="mso-spacerun: yes;">
</span>In order for microbes to grow, they need heat, a moist environment, and
sustenance.<span style="mso-spacerun: yes;"> </span>Incubators provide the perfect
breeding ground.<span style="mso-spacerun: yes;"> </span>TPP prevents the issues
of condensation to build up between dishes, plates, and flasks while stacking.<span style="mso-spacerun: yes;"> </span>TPP dishes plates and flasks are manufactured
with vented stacking feet (Figure 1).<span style="mso-spacerun: yes;">
</span>This not only decreases condensation by even air flow, but also evenly
distributes temperature.<span style="mso-spacerun: yes;"> </span>Even
temperature distribution among multiple samples and treatments is crucial in
maintaining reproducibility.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
<br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh_Po2zkz8m7M15o6rTrUbJp1qTPd8PhXMUIzDXcFMktIFdEGxO4QL5EyRXKHX69D5lJOibYoO2M7FAFgp4sVgxk4XkgkUm6OS3mSziFBUxcfNSwdvHnC5EU7C14pS4yxvxJ1NC7UWifpyq/s1600/Fig+1+blog.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh_Po2zkz8m7M15o6rTrUbJp1qTPd8PhXMUIzDXcFMktIFdEGxO4QL5EyRXKHX69D5lJOibYoO2M7FAFgp4sVgxk4XkgkUm6OS3mSziFBUxcfNSwdvHnC5EU7C14pS4yxvxJ1NC7UWifpyq/s1600/Fig+1+blog.png" /></a></div>
<div class="MsoNormal" style="margin: 0in 0in 10pt; text-indent: 0.5in;">
<v:shape id="Picture_x0020_18" o:spid="_x0000_s1028" style="height: 95.35pt; left: 0px; margin-left: 0px; margin-top: 591.3pt; mso-position-horizontal-relative: margin; mso-position-horizontal: center; mso-position-vertical-relative: margin; mso-position-vertical: absolute; mso-wrap-distance-bottom: 0; mso-wrap-distance-left: 9pt; mso-wrap-distance-right: 9pt; mso-wrap-distance-top: 0; mso-wrap-style: square; position: absolute; text-align: left; visibility: visible; width: 3in; z-index: 251661312;" type="#_x0000_t75"><span style="font-family: Calibri;">
<v:imagedata o:title="" src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image003.png">
<w:wrap anchorx="margin" anchory="margin" type="square">
</w:wrap></v:imagedata></span></v:shape><span style="font-family: Calibri;">An additional
contributor to microbe and mold growth is the location of the treated surfaces
and “deck” seen in some flasks (Figure 2a).<span style="mso-spacerun: yes;">
</span>TPP has a flat growth surface directly up to the neck of the flask.<span style="mso-spacerun: yes;"> </span>In addition, only the bottom surface of the
plates, dishes, and flasks are treated.<span style="mso-spacerun: yes;">
</span>When flasks are moved, media sloshes up onto the “angled deck” of some flasks
providing an ideal breeding ground for microbes and mold.<span style="mso-spacerun: yes;"> </span>The “angled deck” or “dead space” provides
yet another disadvantage: In order to add small amounts of liquid to the flask
with a pipettor, the shaft must go into the neck of the flask to reach the
media (Figure 2b).<span style="mso-spacerun: yes;"> </span>Introducing the shaft
to the neck of the flask opens the flask for contamination.<span style="mso-spacerun: yes;"> </span>TPP flasks, with their lack of dead space, allow
for only the sterile pipette tip to enter the flask for small liquid additions .<span style="mso-spacerun: yes;"> </span>TPP’s innovative design overcomes the
obstacles the “angled deck” presents and prevents yet another contributor to
contamination.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
<br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjyxbbj-ZdXnfwrhPKVTNW_bRVQyVAtFtiagrqYTel8gN1RaNsVkUiiq4GCgNHvbenCBAupEh4VgSlJChl7n3R8tdByp71yKAMbQc6EBujY31DwvOk4av4Sv0Lz9s7ZV_Cfrfb5Vu_OLNRL/s1600/Fig+2+blog.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" height="141" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjyxbbj-ZdXnfwrhPKVTNW_bRVQyVAtFtiagrqYTel8gN1RaNsVkUiiq4GCgNHvbenCBAupEh4VgSlJChl7n3R8tdByp71yKAMbQc6EBujY31DwvOk4av4Sv0Lz9s7ZV_Cfrfb5Vu_OLNRL/s320/Fig+2+blog.png" width="320" /></a></div>
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<span style="font-family: Calibri;"><span style="mso-tab-count: 1;"> </span>The
vented or filtered cap on a flask is meant to prevent contamination or allow
for varying amounts of air to enter.<span style="mso-spacerun: yes;"> </span>The
caps only provide a barrier to contamination when used and secured properly.<span style="mso-spacerun: yes;"> </span>Caps can be easily secured improperly,
slightly skewed when turned on the neck of the flask.<span style="mso-spacerun: yes;"> </span>Improper cap securing can introduce microbes
or mold spores to enter.<span style="mso-spacerun: yes;"> </span><span style="mso-spacerun: yes;"> </span>The design of TPP filtered caps functionally
avoids the issue.<span style="mso-spacerun: yes;"> </span>TPP filtered caps have
a raised ridge area to help line up to ensure they are closed correctly. TPP
vented caps have audible clicks to aid in proper ventilation and the securing
of the cap (Figure 3).<span style="mso-no-proof: yes;"> </span><o:p></o:p></span></div>
<br />
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<v:shape id="Picture_x0020_20" o:spid="_x0000_s1027" style="height: 93.4pt; margin-left: -9pt; margin-top: 48pt; mso-position-horizontal-relative: margin; mso-position-horizontal: absolute; mso-position-vertical-relative: margin; mso-position-vertical: absolute; mso-width-percent: 0; mso-width-relative: margin; mso-wrap-distance-bottom: 0; mso-wrap-distance-left: 9pt; mso-wrap-distance-right: 9pt; mso-wrap-distance-top: 0; mso-wrap-style: square; position: absolute; visibility: visible; width: 2in; z-index: 251662336;" type="#_x0000_t75"><span style="font-family: Calibri;">
<v:imagedata o:title="" src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image005.png">
<w:wrap anchorx="margin" anchory="margin" type="square">
</w:wrap></v:imagedata></span></v:shape><span style="font-family: Calibri;"><span style="mso-tab-count: 1;"> </span></span></div>
<span style="font-family: Calibri;"><br />
<br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjr8H6_j65SF_YVnxIBrJPMqk_4E2kBGSljeGT4IQXwahGX2knaeA35ZJPatRHNnQZk-TB2noQU1ly5RbyBfzyctZ5amLI5SzwWUsu4w8HWJp0EcT7aOXIWhg4OCqW7JbZY8qeKfuO9NYqh/s1600/Fig+3+blog.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjr8H6_j65SF_YVnxIBrJPMqk_4E2kBGSljeGT4IQXwahGX2knaeA35ZJPatRHNnQZk-TB2noQU1ly5RbyBfzyctZ5amLI5SzwWUsu4w8HWJp0EcT7aOXIWhg4OCqW7JbZY8qeKfuO9NYqh/s1600/Fig+3+blog.png" /></a></div>
<br />
<o:p></o:p></span><br />
<br />
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<o:p><span style="font-family: Calibri;"> </span></o:p></div>
<br />
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<o:p><span style="font-family: Calibri;"> </span></o:p></div>
<span style="font-family: Calibri;">Suspension cells are just as easily
affected by contamination as adherent cells.<span style="mso-spacerun: yes;">
</span>TPP manufactures a vessel for suspension cells that not only reduces
contamination, but saves time and risk of contamination by eliminating the need
to transfer to conical tubes before centrifuging .<span style="mso-spacerun: yes;"> </span>Gas exchange is often important in the growth
of suspension cells and can also be a source of contamination.<span style="mso-spacerun: yes;"> </span>TPP bioreactors (Figure 4) have sterile gas
exchange through the 0.22µm filter membrane.<span style="mso-spacerun: yes;">
</span>In addition, five openings of different size within the cap allow for
varying levels of gas permeability, expanding the variety of experim</span><v:shape id="Picture_x0020_3" o:spid="_x0000_s1026" style="height: 167.05pt; left: 0px; margin-left: 0px; margin-top: 0px; mso-position-horizontal-relative: margin; mso-position-horizontal: center; mso-position-vertical-relative: margin; mso-position-vertical: center; mso-wrap-distance-bottom: 0; mso-wrap-distance-left: 9pt; mso-wrap-distance-right: 9pt; mso-wrap-distance-top: 0; mso-wrap-style: square; position: absolute; text-align: left; visibility: visible; width: 109.45pt; z-index: 251664384;" type="#_x0000_t75"><span style="font-family: Calibri;">
<v:imagedata o:title="" src="file:///C:\Users\ELISE~1.AMB\AppData\Local\Temp\msohtmlclip1\01\clip_image007.png">
<w:wrap anchorx="margin" anchory="margin" type="square">
</w:wrap></v:imagedata></span></v:shape><span style="font-family: Calibri;">ents that can be
performed. <span style="mso-spacerun: yes;"> </span>The TPP bioreactor,
available in 15, 50, and 600 mLs not only saves the researcher valuable time
that would be lost due to contamination, but also time lost in transferring for
centrifugation.<o:p></o:p></span><br />
<br />
<div class="separator" style="clear: both; text-align: center;">
<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhWX0o0L4ErKeMm-W1VC6mB6LTZYW0RO_FDa9ECh5eW8t_ce_-R94d7aZ_eIt2ga7dTHfPgsdKLUmlQp40ua1Ry0ZtA6iQzqEEAPBTIK3zg2dFI6VPWw1AhfyuKQMmyyqHaox28RrywG6I7/s1600/Fig+4+blog.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" height="320" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhWX0o0L4ErKeMm-W1VC6mB6LTZYW0RO_FDa9ECh5eW8t_ce_-R94d7aZ_eIt2ga7dTHfPgsdKLUmlQp40ua1Ry0ZtA6iQzqEEAPBTIK3zg2dFI6VPWw1AhfyuKQMmyyqHaox28RrywG6I7/s320/Fig+4+blog.png" width="209" /></a></div>
<div class="MsoNormal" style="margin: 0in 0in 10pt;">
<o:p><span style="font-family: Calibri;"> </span></o:p></div>
<br />
<div class="MsoNormal" style="margin: 0in 0in 10pt; text-indent: 0.5in;">
<span style="font-family: Calibri;">It is essential that every
researcher take extensive steps to ensure their cells and experiments are
performed properly and without error.<span style="mso-spacerun: yes;">
</span>TPP products help prevent contamination and prevent the costly cleanup
and productivity loss.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com1tag:blogger.com,1999:blog-6109992531374028445.post-68023673547832045552013-09-13T07:53:00.001-07:002013-09-13T07:53:46.936-07:00Three-dimensional cell culturing by magnetic levitationN3D hits Nature AGAIN! Contact your MIDSCI representative to try this ground breaking 3D cell culture technology.
Recently, biomedical research has moved toward cell culture in three dimensions to better recapitulate native cellular environments. This protocol describes one method for 3D culture, the magnetic levitation method (MLM), in which cells bind with a magnetic nanoparticle assembly overnight to render them magnetic. When resuspended in medium, an external magnetic field levitates and concentrates cells at the air-liquid interface, where they aggregate to form larger 3D cultures. The resulting cultures are dense, can synthesize extracellular matrix (ECM) and can be analyzed similarly to the other culture systems using techniques such as immunohistochemical analysis (IHC), western blotting and other biochemical assays. This protocol details the MLM and other associated techniques (cell culture, imaging and IHC) adapted for the MLM. The MLM requires 45 min of working time over 2 d to create 3D cultures that can be cultured in the long term (>7 d).
Read more at: http://www.nature.com/nprot/journal/v8/n10/full/nprot.2013.125.htmlMidwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-12635736959680470062013-09-10T14:30:00.001-07:002013-09-10T14:30:31.084-07:00Monoclonal Antibody Production – A discussion on the Culturing of Mouse Hybridoma CellsThe production and maintenance of a hybridoma cell begins with the fusion of a specific antibody producing B cell, to a cancer B cell called a myeloma, which does not produce an antibody by itself. Fusion results in an immortalized line called a hybridoma that will faithfully produce a specific antibody against a single epitope called a monoclonal antibody. Once produced, proper maintenance and culturing is required to maximize the performance and continued production of the antibody in question. These were the topics covered in our last Ask the Expert session – Monoclonal antibody production and the culturing of mouse hybridoma cells.
Dr. Fawcett is Director of the BioTechnical Institute of Maryland (BTI) a non-profit institute located in Baltimore, Maryland. He is also the Founder and Director of BioSciConcepts, a social venture of BTI that provides hands-on training for professional scientists in cell culture, baculovirus based expression, as well as topics such as molecular biology, PCR and real-time PCR. Dr. Fawcett who has been in biotechnology for over 30 years, provided real hands-on suggestions that readers could benefit from right away.
Question topics included:
Use of azaguanine
Cell viability post cryopreservation
Purity of antigen
Serum-free culture
Problem obtaining hybridomas in spite of strong signal
Culture Conditions
Rat-mouse hybridomas
Read the answers here: http://cellculturedish.com/2013/07/monoclonal-antibody-production-a-discussion-on-the-culturing-of-mouse-hybridoma-cells/ Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com4tag:blogger.com,1999:blog-6109992531374028445.post-78635766188090378282013-08-29T11:32:00.001-07:002013-08-29T11:32:02.815-07:00All About CHO – A discussion on everything from the basics to troubleshootingWhile CHO cells are a popular choice for biopharmaceutical manufacturing, they can still present a challenge to work with. With so many cell culture scientists regularly searching for ways to improve on product yield, product quality, cost, and a myriad of other manufacturing challenges faced with biopharmaceutical manufacturing, we felt that CHO cell production was a great topic to pursue.
Question topics included:
Why CHO cells are so popular in biomanufacturing
Different types of CHO cells
CHO genome sequence
Shipping requirements
Lactic acid build up in culture
Check out the article here: http://cellculturedish.com/2013/06/all-about-cho-a-discussion-on-everything-from-the-basics-to-troubleshooting/ Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-47474965795266242222013-08-27T17:15:00.001-07:002013-08-27T17:15:34.755-07:00New and Improved Cultureware is Designed with the Scientist in MindIn the past cultureware has mostly been focused on culturing standard cell lines that have historically been easy to culture. However, with the explosion of stem cell culture and the now common practice of culturing many different cell types, cultureware has needed to evolve as well. See how Techno Plastic Products (TPP) have innovated their products for these changes.
http://cellculturedish.com/2013/07/new-and-improved-cultureware-is-designed-with-the-scientist-in-mind/ Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-91957876230432940382013-08-15T06:48:00.000-07:002013-08-15T06:48:40.853-07:00New Vaccines- Coming Soon to a Doctor's Office Near You...<span style="background-color: white; color: #444444; font-family: helvetica, arial, sans-serif; font-size: 14px; line-height: 20px;">These new vaccines hold much promise in improving public health and can positively impact many lives. Check out the new vaccines in this interesting article by The Cell Culture Dish: </span><div>
<a href="http://cellculturedish.com/2012/07/new-vaccines-coming-soon-to-a-doctors-office-near-you/">http://cellculturedish.com/2012/07/new-vaccines-coming-soon-to-a-doctors-office-near-you/</a></div>
Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-31704225134985147822013-08-08T11:27:00.003-07:002013-08-08T11:27:51.822-07:00Your Questions Answered - "Ask the Expert" on The Cell Culture Dish<span style="background-color: white; color: #444444; font-family: helvetica, arial, sans-serif; font-size: 14px; line-height: 20px;">Many of the common cell types we use are robust and grow on cell culture treated plastic. However there are many cell types that have difficulty attaching and/or spreading on cell culture treated plastic. In other cases, cells may attach but not differentiate unless the attachment surface correct. To learn more about matrices or if you are using them and have questions, this Ask The Expert topic is your chance to learn more about attachment surfaces and the rationale for using them.</span><br />
<span style="background-color: white; color: #444444; font-family: helvetica, arial, sans-serif; font-size: 14px; line-height: 20px;"><br /></span>
<span style="background-color: white; color: #444444; font-family: helvetica, arial, sans-serif; font-size: 14px; line-height: 20px;">Check out what people are asking: </span><span style="background-color: white; color: #666666; font-family: Georgia, serif; font-size: 13px; line-height: 20.796875px;"> </span><a href="http://cellculturedish.com/2013/08/matrices-for-cell-culture-how-to-develop-healthy-attachment/" style="font-family: Georgia, serif; font-size: 13px; line-height: 20.796875px;">http://cellculturedish.com</a>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-63376083101290244432013-08-01T10:08:00.001-07:002013-08-01T10:08:26.584-07:00Your Questions Answered - "Ask the Expert" on The Cell Culture Dish<p>In the past cultureware has mostly been focused on culturing standard cell lines that have historically been easy to culture. However, with the explosion of stem cell culture and the now common practice of culturing many different cell types, cultureware has needed to evolve as well. Over the years, improvements have been developed to increase the success of cultureware and to provide a more hospitable cell-growing environment, including innovations like gas-plasma treatment and biological extracellular matrices. Yet there is still opportunity to improve cultureware and address some of the most common challenges cell culture scientists face.</p>
<p>Check out what people are asking about their cultureware on <a href="http://cellculturedish.com/ask-the-expert/innovations-in-cultureware/">"Ask the Expert" at The Cell Culture Dish</a></p>
<p> </p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-66126266988254671012013-07-22T13:20:00.001-07:002013-07-22T13:20:34.348-07:00MIDSCI featured in "Ask the Expert" on The Cell Culture Dish<p>Our own Graziella Mendonsa, PhD is participating in this week's "Ask the Expert" on The Cell Culture Dish. Please visit http://ow.ly/ncxOy to participate!</p>
<p>We'd love to hear from you!</p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-89982305694184026242013-07-18T11:09:00.001-07:002013-07-18T11:09:43.825-07:00Using the iPhone/iPod to Automate Cytotoxicity Testing with the BiO Ass<p>Have you ever used your iPhone/iPod with your research? What about with your 3D cell culture work?</p>
<p>N3D Biosciences is launching their BiO Assay to do just that! Check it out!</p>
<p>http://www.n3dbio.com/wp-content/uploads/2013/05/Using-the-iPhone-to-Automate-Cytotoxicity-Testing-with-the-BiO-Assay.pdf</p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-65755828551061894592013-07-18T11:08:00.001-07:002013-07-18T11:08:02.366-07:00Using the iPhone/iPod to Automate Cytotoxicity Testing with the BiO AssayHave you thought about using your iPhone/iPod for your research? Look at the new product N3D Biosciences is launching to do just that. Great stuff!
http://www.n3dbio.com/wp-content/uploads/2013/05/Using-the-iPhone-to-Automate-Cytotoxicity-Testing-with-the-BiO-Assay.pdfMidwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-49505977367451112072013-07-12T06:25:00.001-07:002013-07-12T06:25:24.314-07:00New and Improved Cultureware is Designed with the Scientist in Mind<p>Check out our guest blog on The Cell Culture Dish written by our own Graziella Mendonsa, Ph.D.</p>
<p>A Guest Blog by Graziella Mendonsa, Ph.D., Product Manager at MIDSCI</p>
<p>http://cellculturedish.com/2013/07/new-and-improved-cultureware-is-designed-with-the-scientist-in-mind/</p>
<p><img src="http://cellculturedish.com/2013/07/new-and-improved-cultureware-is-designed-with-the-scientist-in-mind/" alt="TPP Cultureware" /></p>
<p>Introduction</p>
<p>In the past cultureware has mostly been focused on culturing standard cell lines that have historically been easy to culture. However, with the explosion of stem cell culture and the now common practice of culturing many different cell types, cultureware has needed to evolve as well. Over the years, improvements have been developed to increase the success of cultureware and to provide a more hospitable cell-growing environment, including innovations like gas-plasma treatment and biological extracellular matrices. Yet there is still opportunity to improve cultureware and address some of the most common challenges cell culture scientists face. Techno Plastic Products (TPP) is a Premium Swiss manufactured line of cell cultureware that is designed by scientists for scientists. Virgin plastics of highest purity and quality are poured with perfection into uniquely engineered molds to deliver the perfect flask, dish or plate for adherent cells to attach to. Suspension cells also get an updated housing with a conical Bioreactor that significantly enhances distribution of gas exchange and nutrition. Quality control is the number one priority for TPP, which includes the testing of each and every piece manufactured at their site.</p>
<p>Cultureware Challenges</p>
<p>Maintaining Standard Temperatures</p>
<p>Cell culture in general is treated as a great in vitro model system to assay signal transduction, cellular cross talk and responses to externally applied gene activators or inhibitors. Since cells in culture are sensitive to environmental stimuli and react accordingly for adaptation, it is essential to maintain their necessary vitals such as temperature and CO2 stable and non-fluctuating. Almost every lab with multiple scientists performing cell culture usually stacks their flasks, dishes and plates to save on space in their incubator. However, a key question to ask is “Are the vessels in the middle of the stack the same temperature as that on the top or bottom of the stack?”. The answer is a resounding “Probably Not!.” Therefore TPP has engineered a raised growth surface on their flasks, dishes and plates to avoid insulation of the adjoining vessels and has designed the vessels to have a stable footing for enhanced security in stacking. Maintaining Optimal Nutrition, Gas Exchange and Gene Expression The foundation of cell culture at the time of seeding in a flask or dish, undoubtedly sets up for consequent cellular viability and propagation during passaging and for consistent downstream gene reporting assays. Therefore how cells are initially thawed from frozen and plated during the first 2-3 days has a dramatic impact on cellular behavior downstream. Therefore controlling the flatness of the surface upon which cells adhere to reigns important to obtain consistent cell monolayers. Cells that are growing in layers are not only deprived of nutrition and the optimal gas exchange, but are also now transcribing uneven gene expression. TPP manufacturers one of the flattest growth surfaces in the market.</p>
<p>Evaporation of Media</p>
<p>Evaporation of media in 96-well plates appears to be one of the most common complaints from cell culture scientists. This is due to the overheating of the outer wells and a consequent temperature gradient across the well plate and across the stack of well plates in the incubator. This uneven heating can also lead to condensation build up which are breeding grounds for bacteria. The temporary solution generically recommended is to fill the outer wells with media alone or PBS. However the wastage of unused wells for cell growth and experimentation amounts to almost 40%. TPP plates eliminate this concern with its raised growth surface for optimal stackability and consequent even heating from all sides. Ultimately, a 96-well plate is used for multiple replicates of control and experimental treatments and with TPP plates, one can achieve that goal because surrounding environmental conditions such as temperature are no longer a contributor to variation.</p>
<p>Eliminating Dead Space</p>
<p>Focusing in on the flasks, it is strikingly obvious that TPP flasks are shorter in length. That was purposely designed to encompass the entire bottom of the flask as the treated growth surface. Therefore, with no wasted “dead space” in flask, the cell to media ratio is now optimally maximized. All TPP flasks come with an angled neck; this small design tweak serves as the best method to eliminate media accumulation in the neck and thus the consequent high risk of contamination. TPP also has filtered caps for their flasks that are designed to be hydrophobic in nature, so that incase media does slosh against the cap when the flask is either laid at the microscope or put back in the incubator, the risk of contamination is minimized greatly. The angled neck also provides full access to the internal corners of the flask for a serological pipette or scraper during trypsinization or cell harvestation respectively. Summary TPP dishes have ingenuity spelled all over it. From the unique grip ring on the edge of the dish that prevents accidental dropping to the internal clock orientation for visual quadrants during microscopy, TPP continues to invent upgrades to the cell culture dish that take cell cultureware from standard to ergonomic. TPP cultureware, designed by scientists for scientist, provide you with unique and innovative upgrades that translate to more consistent seeding, handling, treatment and harvestation of cells. Peace of mind included.</p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-77580973758133311912013-07-01T07:03:00.001-07:002013-07-01T07:03:44.554-07:00And the JUNE BACKPACK WINNER IS... <p>Our JUNE BACKPACK WINNER is... Benjamin Goldschmidt from University of MO - Columbia!! Congrats!! Your rep, Nick B, will personally deliver it to you. Thanks everyone for participating! For your chance to win, fill out our survey at http://ow.ly/mxIF8. We draw winners every month. :)</p>
<p><img src="http://ow.ly/mxNlq" alt="backpack winner" /></p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-17536918320663184702013-06-27T14:19:00.001-07:002013-06-27T14:19:01.035-07:00See what we're up to! Visit us on social media!<p>Find the latest MIDSCI deals and promos, science news, contests, trivia, and more on our social media sites!</p>
<p>Like Us on <a title="MIDSCI Facebook" href="https://www.facebook.com/Midsci" target="_self">FACEBOOK </a></p>
<p>Connect With Us on <a title="MIDSCI LinkedIn" href="http://www.linkedin.com/company/118644?trk=tyah" target="_blank">LINKEDIN </a></p>
<p>Be Our Fan on <a title="MIDSCI Twitter" href="https://twitter.com/MIDSCI" target="_blank">TWITTER </a></p>
<p>View Our Videos on <a title="MIDSCI YouTube" href="https://www.youtube.com/channel/UCgcZNxrmuU5iuVWPeWYtj9g" target="_blank">YOUTUBE</a></p>
<p><img src="http://bit.ly/126VXk7" alt="MIDSCI iphone" width="339" height="339" /></p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-73505170538016696572013-06-19T12:29:00.001-07:002013-06-19T12:29:55.113-07:00Shout out to Dr. Peter Smith & Dr. Anuradha Chakrabarty for their newest publication! Way to go! <p><strong>Researchers at KU Medical Center identify a new target for treating pain</strong></p>
<p><em>June 14, 2013 </em></p>
<p><em>By David Martin</em> </p>
<p>A hormone that has been used to control blood pressure may hold the key to a new approach for treating pain, according to research at the University of Kansas Medical Center. The hormone, angiotensin II, constricts blood vessels and releases a substance that causes the body to retain salt and water. KU neuroscientist Peter Smith, Ph.D., and his colleagues have found that blocking angiotensin II receptors prevents the increase in sensitivity that normally accompanies inflammation. The mechanics of the angiotensin II system's ability to regulate pain pathways was not appreciated before, Smith says.</p>
<p>The study has been published online in <a href="http://www.jpain.org/article/S1526-5900%2813%2900959-0/abstract">The Journal of Pain</a>. </p>
<p>The finding is based on a previous study of estrogen that Smith, professor of molecular and integrative physiology and director of the Institute for Neurological Discoveries at KU, published in 2008. "We know that the hormone estrogen makes women more prone to many painful conditions, and we found that estrogen actually caused some pain-sensing nerve cells to grow," he says, adding that many chronically painful conditions are accompanied by an increase in pain-sensing nerves.</p>
<p>In looking at what genes were affected by estrogen, Smith and his colleagues were surprised to learn that nerve cells started making more of a protein that allows cells to respond to angiotensin II. This led the researchers to speculate that the protein, angiotensin II receptor type 2, or AT2, might be important to pain.</p>
<p>Tests on rodents confirmed the relationship. In the experiment, rats' hind paws were injected with an irritant. Exposure to touch and heat revealed the paws' sensitivity.</p>
<p>Some of the rats received a chemical known to inhibit AT2. In these rats, angiotensin II did not cause pain-sensing nerves to sprout, and paw sensitivity was the same as in rats that did not receive the irritant. In short, the AT2 blocker uncoupled the link between inflammation and the nerve response.</p>
<p>"It appears that AT2 is critical for the sensations of pain, and that AT2 receptor inhibitors are about as effective as morphine - but without all of the dangerous side effects," says senior scientist Anuradha Chakrabarty, Ph.D., the paper's first author. Zhaohui Liao, M.D., also contributed to the study.</p>
<p>The study, Smith says, confirms that the proliferation of pain-sensing nerves associated with chronic pain syndromes is meaningful. "More sprouting means more pain," he says.</p>
<p>The study is the first to shed light on the mechanism of overgrowth of pain-sensing nerves, Smith says. Inhibiting AT2, he adds, "represents one of the few drug therapies that actually is known to be directed at the underlying biological mechanisms that may be responsible for some aspects of chronic pain."</p>
<p>Chronic pain has been estimated to cost the U.S. between $560 and $635 billion in health care costs and lost productivity. Nonsteroidal anti-inflammatory drugs may not work for all patients and have the potential to damage the tissue of the gastrointestinal tract, kidney and liver. Opiates pose the risk of addiction and overdose and are unsuitable for long-term treatment of chronic pain.</p>
<p>An Australian pharmaceutical company is currently testing an AT2 receptor blocker in a clinical trial with patients with postherpetic neuralgia, nerve pain caused by shingles.</p>
<p>The KU study was supported by the National Institutes of Health grant RO1HD049615.</p>
<p><em>Article found at <a href="http://ow.ly/mcdSL">http://ow.ly/mcdSL</a></em></p>Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-52412387127787846782013-03-01T10:48:00.001-08:002013-03-01T10:48:31.206-08:00February Backpack winnerCongrats to our February Backpack winner: Sean Blake. Your Midsci Rep Nick Bouyoukos will be delivering your prize.Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-41380569714127957992013-02-01T08:43:00.001-08:002013-02-01T08:43:26.602-08:00January winnerCongratulations to January's backpack winner Ben Winkler for sending his customer survey in. Your Midsci rep Peter Cain will drop off your gift!Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0tag:blogger.com,1999:blog-6109992531374028445.post-19781119112523065372013-01-02T07:37:00.002-08:002013-01-02T07:37:12.227-08:00December 2012 Backpack winner!Congratulations to Bin Wang at University of Illinois Chicago for being December's backpack winner of the month. Your Midsci Rep Caroline will be delivering your gift :)Midwest Scientific St.Louishttp://www.blogger.com/profile/04362363897000519440noreply@blogger.com0