MIDSCI now carries new Expedeon® RunBlue™ Pre-Cast gels!
Check out our options
to fit your unit today!
Seven tips to improving your Western Blots:
· 1. Uniformly distributed high background?
o
You may have insufficiently washed. Increase the number of washes and buffer
volume.
§
Make washing easier with Blot
Boxes.
o
Antibody concentrations too high
§
Make sure to optimize your antibody
concentrations by running strips at varying concentrations to determine best
concentration for your samples.
· 2. Weak or no signal?
o
Your protein may have not transferred well.
§
Check your transfer buffer make-up and blotting
procedure. Stain gel to visualize
proteins remaining in gel.
· 3. The Ladder Lane is black
o
The antibody may be reacting with your MW
(ladder) marker.
§
Add an empty lane between the MW marker and
sample lane.
· 4. The band of interest is too low/too high on the
blot.
o
Separation is not efficient.
§
Change the gel percentage: A higher percentage
for small protein, a lower percentage for larger proteins. Or try a gradient
gel.
· 5. Ripped resolving gel when moving to the cassette
for transferring?
o
Try Run Blue™ pre-cast gels from MIDSCI. They are stronger and resist tearing! No more
piecing together torn gels!
· 6. Sample bleeding over to another well or
difficulty loading?
o
Try the deeper wells from Expedeon! Easily hold 35ul of sample without bleeding
over to another well!
o
Try gel-loading
tips. Available in flat or round,
they make gel loading easier!
· 6. Worried about pre-cast gels expiring or losing
resolution before you can use them?
o
Expedeon Run Blue™ gels have a 2 year shelf life
with no loss in resolution. Use them
confidently from purchase date to 2 years later!
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